Apresentação em tema: "Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables."— Transcrição da apresentação:
1 Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, Campus de Gualtar, Braga, PortugalIBB- Institute for Biotechnology and Bioengineering, Braga, Portugal
2 3B´s RESEARCH GROUP Staff - Rui L. Reis (Director) - João F. Mano - Nuno M. Neves- Natália AlvesOther PhD HoldersAlexandra MarquesAna GrenhaAna FriasAntónio SalgadoCarlos ViegasErkan BaranGabriela SilvaHelena AzevedoIsabel DiasIva PashkulevaJohan BeneschLuciano BoeselMaria Manuela GomesMazen TamerRicardo PiresRita SerraRui Amandi de SousaSandra SampaioSusana MartinsTiago SilvaZhongkui HongPhD Students/ Other Full time ResearchersAdriano PedroAlbino MartinsAna MendesAna PinheiroAna OliveiraAna SousaAna PintoAnabela PintoBarbara BertiniBrecht ClaesBruno FerreiraCatarina AlvesCornelia BrunnerDaniela CoutinhoElisabete PinhoElizabeth BalmayorEmanuel FernandesHelena LimaIsabel LeonorIvo ArosoJessica GrechJoana MagalhãesJoão OliveiraJoaquim FerreiraJoaquim OliveiraJosé RodriguezJosé PereiraJosé JúniorKadriye TuzlakogluLeandro GardelMárcia RodriguesMarina SantosMarta SilvaPatrícia BaptistaPaula SolPaula PérezPaulo BessaRicardo SilvaRogério SousaRosa BessadaSandra OsórioSangwong ChungSatyabrata GoshSílvia PereiraSimone SilvaSusana SantosTeresa SilvaTírcia SantosTommaso RadaVitor Espírito SantoVítor SilvaWojciech SzymczykTechnical StaffAriana SantosBárbara BarrosoBerta AlmeidaFilipe RibeiroLiliana GomesRicardo GomesTânia Delalande AlvesVirgínia Araújo
3 TE: AN INTERDISCIPLINARY SCIENCE “Tissue Engineering (TE) is an interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function”Langer and Vacanti., Science 260, 920 (1993)BiologyBiochemistryChemistryMaterials Science& engineeringMedical scienceTE
4 Biodegradable polymers Porous/interconected Which processing method?? CONSTRUCTION OF HUMAN TISSUE SUBSTITUTESWhat material??Biodegradable polymersWhich structure ??Porous/interconectedWhich processing method??SCAFFOLDcells+ Growth factors
5 Starch + EVOH or CA or PCL or PLA Materials used at the 3B’s GroupStarch + EVOH or CA or PCL or PLAHigh potential for a all range ofbiomedical applications:- tissue engineering scaffolding- drug delivery carrier systems- bone replacement / bone defects; bone plates / screws; partially degradable bone cementsChitosan based materialsReferring to polymeric materials, just a short list is presented here on polymers have been proposed for the construction of the scaffold in tissue engineering application, spitted into two major classes: synthetic polymers and natural based-ones. We believed that the combination of both types of polymers could be a possibility for combining the most favourable properties of synthetic and natural polymers, allowing to tailor the final materials properties and processability, together with the possibility to attach biologically functional groups that promote favourable cell-polymer interactions.other polysaccharides, proteins and blends with biodegradable polyesters…
6 3B´s SCAFFOLD PROCESSING ROUTES Injection moulding with blowing agentsExtrusion with blowing agentsMicrowave BakingFiber Bonding (Wet or Melt-spinning)Compression moulding and salt leachingSolvent casting-particle leachingIn-situ polymerization(In-situ) Enzymatic DegradationFreeze dryingRapid prototyping………Using all these materials our group have developed and optimize a very wide range of processing technologies to obtain scaffolds with different porous morphologies and different properties. Some of these methodologies were based in conventional processing technologies, such as injection molding and extrusion, and other were based on more non-conventional technologies. In the rest of the talk I will try to give some examples of how chitosan could be shaped into different forms.
7 ceramic+chitosan bilayered scaffolds HA slurrypore size: 50 – 350 µmchitosan solutionburningpolyurethane spongefreeze-dryingJust an example in this context combining ceramics and chitosan. First a polyurethane sponge is impregnated with a HA slurry. A ceramic scaffold may be then obtained after burning this ensemble. The scaffold is placed in a soft mould when a viscous chitosan solution is added into the top. After freeze drying a bilayer scaffold is obtained with the bottom layer being adequate to integrate with bone and the chitosan scaffold to the cartilage side. Studies are in progress to develop special bioreactor that may allow for the co-culturing of ostheoblasts and chondrocites in the two compartments.
9 Cell response to bilayered scaffolds 20 mcartilage regioncondrogenic mediumgoat marrow cells28 days100 µmosteogenic mediumIsabel Dias, Carlos Viegasbone region14 days
10 Human Osteoblast-like cells (SaOs-2) and human fibroblasts (MRC-5) Cell encapsulation and co-cultures+-PolyanionCa2+ ionsCellsHuman Osteoblast-like cells (SaOs-2) and human fibroblasts (MRC-5)sodium alginate + Iota carrageenan+ cellsCaCl2+NaClFibroblastsOsteoblasts
11 INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLES SaOs-2 with CMC/PAMAM-FITCafter 14 daysnovel carboxymethyl-chitosan/poly (amidoamine) (CMC/PAMAM) dendronized polymers (natural-based polymers + dendrimers).Slide 9Falar que a solução oxidante foi abandonada e usou-se a solução ácida para preparar os materiais porosos.
12 NEW JOURNAL - TERM EU FUNDED PROJECTS Editor-in-Chief PROTEUS STREP – WaCheUpIP-GENOSTEMNANOPHOTOTECHMarie Curie EST JoinTEdMarie Curie: POLYSTEMMarie Curie EST - ALEA JACTA ESTSTREP – HIPPOCRATESPROTEUSINTERREG IIIANoE – EXPERTISSUESMarie Curie SCF - InVENTSNEW JOURNAL - TERMEditor-in-ChiefProf. Rui L. Reis
13 Biomaterials Biodegradables Biomimetics email@example.com Concluding, we believe that natural-origin polymers may be a potential source of materials to be used in tissue engineering. However, much work is still needed, namely in controlling the chemical structure and the morphology at different length scales, to optimise the interaction between the scaffolds and the cells and tissues.