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Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables.

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Apresentação em tema: "Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables."— Transcrição da apresentação:

1 Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, Campus de Gualtar, Braga, Portugal IBB- Institute for Biotechnology and Bioengineering, Braga, Portugal

2 PhD Students/ Other Full time Researchers Adriano Pedro Albino Martins Ana Mendes Ana Pinheiro Ana Oliveira Ana Sousa Ana Pinto Anabela Pinto Barbara Bertini Brecht Claes Bruno Ferreira Catarina Alves Cornelia Brunner Daniela Coutinho Elisabete Pinho Elizabeth Balmayor Emanuel Fernandes Helena Lima Isabel Leonor Ivo Aroso Jessica Grech Joana Magalhães João Oliveira Joaquim Ferreira Joaquim Oliveira José Rodriguez 3B´s RESEARCH GROUP Staff - Rui L. Reis (Director) - João F. Mano - Nuno M. Neves - Natália Alves José Pereira José Júnior Kadriye Tuzlakoglu Leandro Gardel Márcia Rodrigues Marina Santos Marta Silva Patrícia Baptista Paula Sol Paula Pérez Paulo Bessa Ricardo Silva Rogério Sousa Rosa Bessada Sandra Osório Sangwong Chung Satyabrata Gosh Sílvia Pereira Simone Silva Susana Santos Teresa Silva Tírcia Santos Tommaso Rada Vitor Espírito Santo Vítor Silva Wojciech Szymczyk Technical Staff Ariana Santos Bárbara Barroso Berta Almeida Filipe Ribeiro Liliana Gomes Ricardo Gomes Tânia Delalande Alves Virgínia Araújo Other PhD Holders Alexandra Marques Ana Grenha Ana Frias António Salgado Carlos Viegas Erkan Baran Gabriela Silva Helena Azevedo Isabel Dias Iva Pashkuleva Johan Benesch Luciano Boesel Maria Manuela Gomes Mazen Tamer Ricardo Pires Rita Serra Rui Amandi de Sousa Sandra Sampaio Susana Martins Tiago Silva Zhongkui Hong

3 Tissue Engineering (TE) is an interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function Langer and Vacanti., Science 260, 920 (1993) TE: AN INTERDISCIPLINARY SCIENCE Medical science Biology Biochemistry Materials Science & engineering Chemistry TE

4 cells + Growth factors CONSTRUCTION OF HUMAN TISSUE SUBSTITUTES What material?? Biodegradable polymers Which structure ?? Porous/interconected Which processing method?? SCAFFOLD

5 Materials used at the 3Bs Group Starch + EVOH or CA or PCL or PLA High potential for a all range of biomedical applications: - tissue engineering scaffolding - drug delivery carrier systems - bone replacement / bone defects; bone plates / screws; partially degradable bone cements Chitosan based materials other polysaccharides, proteins and blends with biodegradable polyesters…

6 3B´s SCAFFOLD PROCESSING ROUTES Injection moulding with blowing agents Extrusion with blowing agents Microwave Baking Fiber Bonding (Wet or Melt-spinning) Compression moulding and salt leaching Solvent casting-particle leaching In-situ polymerization (In-situ) Enzymatic Degradation Freeze drying Rapid prototyping ………

7 ceramic+chitosan bilayered scaffolds polyurethane sponge HA slurry burning chitosan solution freeze-drying pore size: 50 – 350 µm

8 STEM /PROGENITOR CELLS EMBRYONIC BONE MARROW ADIPOSE TISSUE UMBILICAL CORD AMNIOTIC MEMBRANE

9 Cell response to bilayered scaffolds 100 µm 20 m cartilage region bone region condrogenic medium osteogenic medium goat marrow cells 28 days 14 days Isabel Dias, Carlos Viegas

10 Human Osteoblast-like cells (SaOs-2) and human fibroblasts (MRC-5) Cell encapsulation and co-cultures Osteoblasts Fibroblasts CaCl 2 +NaCl sodium alginate + Iota carrageenan + cells Polyanion Ca 2+ ions Cells

11 SaOs-2 with CMC/PAMAM-FITC INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLES after 14 days novelcarboxymethyl-chitosan/poly (amidoamine) (CMC/PAMAM) dendronized polymers (natural-based polymers + dendrimers). novel carboxymethyl-chitosan/poly (amidoamine) (CMC/PAMAM) dendronized polymers (natural-based polymers + dendrimers).

12 EU FUNDED PROJECTS STREP – HIPPOCRATES Marie Curie EST - ALEA JACTA EST NoE – EXPERTISSUES Marie Curie SCF - InVENTS STREP – WaCheUp IP-GENOSTEM NANOPHOTOTECH Marie Curie EST JoinTEd Marie Curie: POLYSTEM PROTEUS INTERREG IIIA NEW JOURNAL - TERM Editor-in-Chief Prof. Rui L. Reis

13 Biomaterials Biodegradables Biomimetics


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