Imunogenicidade das proteinas Cry? Vazquez-Padron RI, Moreno-Fierros L, Neri-Bazan L, De La Riva GA, Lopez-Revilla R. Intragastric and intraperitoneal.

Apresentação em tema: "Imunogenicidade das proteinas Cry? Vazquez-Padron RI, Moreno-Fierros L, Neri-Bazan L, De La Riva GA, Lopez-Revilla R. Intragastric and intraperitoneal."— Transcrição da apresentação:

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3 Imunogenicidade das proteinas Cry? Vazquez-Padron RI, Moreno-Fierros L, Neri-Bazan L, De La Riva GA, Lopez-Revilla R. Intragastric and intraperitoneal administration of Cry1Ac protoxin from Bacillus thuringiensis induces systemic and mucosal antibody responses in mice. Life Sci 1999;64:1897-1912. 2.Vazquez-Padron RI, Moreno-Fierros L, Neri-Bazan L, Martinez-Gil AF, de-la-Riva GA, Lopez-Revilla R. Characterization of the mucosal and systemic immune response induced by Cry1Ac protein from Bacillus thuringiensis HD 73 in mice. Braz J Med Biol Res 2000;33:147-155. 3Guerrero GG, Dean DH, Moreno-Fierros L. Structural implication of the induced immune response by Bacillus thuringiensis Cry proteins: role of the N-terminal region. Mol Immunol 2004;41:1177-1183. 4Vazquez RI, Moreno-Fierros L, Neri-Bazan L, De La Riva GA, Lopez-Revilla R. Bacillus thuringiensis Cry1Ac protoxin is a potent systemic and mucosal adjuvant. Scand J Immunol 1999;49:578-584. 5Esquivel-Perez R, Moreno-Fierros L. Mucosal and systemic adjuvant effects of cholera toxin and Cry1Ac protoxin on the specific antibody response to HIV-1 C4/V3 peptides are different and depend on the antigen co-administered. Viral Immunol 2005;18:695-708. 6Moreno-Fierros L, Ruiz-Medina EJ, Esquivel R, Lopez-Revilla R, Pina-Cruz S. Intranasal Cry1Ac protoxin is an effective mucosal and systemic carrier and adjuvant of Streptococcus pneumoniae polysaccharides in mice. Scand J Immunol 2003;57:45-55. 7Guimaraes VD, Drumare MF, Ah-Leung S, Lereclus D, Bernard H, Creminon C et al. Comparative study of the adjuvanticity of Bacillus thuringiensis Cry1Ab protein and cholera toxin on allergic sensitisation and elicitation to peanut. Food and Agricultural Immunology 2008;19:325- 337. Traavik, T. SBPC, 2010

4 Mudanças em produtos transgênicos? Rosati, A., Bogani, P., Santarlasci, A., Buiatti M. Characterization of 3’ transgenes insertion site and derived mRNAs in MON810 Yieldgard maize. Plant Mol Biol 67: 271-281, 2008 Prescott VE, Campbell PM, Moore A, Mattes J, Rothenberg ME, Foster PS, Higgind TJV, Hogan SP. Transgenic expression of bean alpha-Amylase inhibitor in peas results in altered structure and immunogenicity J. Agric. Food Chem. 53: 9023-9030, 2005 Prescott VE and Hogan SP. Genetically modified plants and food hypersensitivity diseases: usage and implications of experimental models for risk assessment. Pharmacology and Therapeutics 111: 374-383, 2006 Zolla L et al. Proteomics as a complementary tool for identifying unintended side effects occurring in transgenic maize seeds as a result of genetic modification. Journal of Proteome Research 7: 1850-1861, 2008 Myhre, MR, Fenton KA, Eggert J, Nielsen KM, Traavik T. 2006. The 35 S CaMV plant virus promoter is active in human enterocyte-like cells. Eur Food Res Tech 222:185-193 Traavik, T. SBPC, 2010

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6 Proteínas alteradas em ervilhas GM podem tê-las tornado mortais Agricultural Food Chemistry, 2005 Os fracassos…

7 Spot no. 134 exclusivo em Bt (Campos Novos)

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9 Residuos e metabólitos do glifosato e também da toxina Cry1Ac foram enncontrados em sangue de mulheres não-grávidas, grávidas e em fetos.

10 Riscos não estão relacionados ao que os cientistas sabem, mas ao que eles não sabem. Riscos estão associados a incertezas. Caruso, D. Intervention. San Francisco, Hybrid Vigor Press, 2006, 252p. RISCO..... Pois é no contexto da incerteza que viceja a esperança, o juízo e a valoração da subjetividade, capaz de concretizar o inusitado. Lieber, RR. & Romano-Lieber NS. Risco, incerteza e as possibilidades de ação na saúde ambiental. Rev. Bras. Epidemiol., 6(2):121-34, 2003.

11 Milho T25 (LL25) – 2008 Milho MON 810 (Cry1Ab) – 2008 Milho BT 11 (Cry1Ab, PAT) – 2008 Milho NK 603 (m epsps) Milho GA 21 (m epsps) Milho TC 1507 (Cry1F) Milho BT 11 x GA21 (Cry1Ab, mepsps) Milho MON 810 x NK603 (Cry1Ab, m epsps) Milho MIR 162 (Vip3A) Milho MON 89034 (Cry1A.105, Cry2Ab) Milho TC 1507 x NK603 (Cry1F, m epsps) Milho MON 89034 × TC1507 × NK603 ( Cry1A.105, Cry2Ab, Cry1F, pat, mpsps) Milho MON 88017 ( Cp4 epsps, cry3Bb1 ) Milho MON 89034 x NK 603 ( Cry1A.105, Cry2Ab, mpsps) Milho Bt11xMIR162XGA21 ( Cry1Ab, pat, Vip3A, mpsps) Eventos de milho Transgênico aprovados no Brasil

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16 Obrigado rubens.nodari@ufsc.br