ITRANS database Sorting structural motifs in cellular transporters Afonso M.S. Duarte Biological Energy Transduction Lab ITQB-UNL, Portugal.

Apresentação em tema: "ITRANS database Sorting structural motifs in cellular transporters Afonso M.S. Duarte Biological Energy Transduction Lab ITQB-UNL, Portugal."— Transcrição da apresentação:

1 ITRANS database Sorting structural motifs in cellular transporters Afonso M.S. Duarte Biological Energy Transduction Lab ITQB-UNL, Portugal

2 Cellular membrane transporters Takamori et al, Cell. 2006 Nov 17;127(4):831-46 Nutrients uptake “Waste disposal” Signal transduction Energy production Drug targets

3 Ion transporters Activity Regulation Molecular mechanism Protein structure at different levels Atomic resolution Structural motifs Repeats

4 What are the basic features of repeats ? Can repeats be systematized and characterized at sequence and structural level? ?? ? ? ? ? Structural motifs and repeats

5 Structural motifs Repeats transport mechanism inhibition activation Post-translational modifications # TMs #  # β # kinks ions repeat orientation Ion transporters Charges hydrophobicity Itrans DB

6 Itrans – Data sources Itrans DB literature DB of ion and small molecules transporters with known 3D structure

7 Itrans – Structure

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9 Itrans – input

10 Itrans – full table ≠ query –> custom-made tables

11 Itrans – repeats table

12 Itrans – transporter entry

13 Itrans - Statistics

14 Acknowledgments ITQB-UNL Manuela Pereira Guilherme Benedito José Silva Dalila Fernandes Joana Simões Cátia Costa Ana Batista Bruno Marreiros Filipa Calisto Filipa Sena Patrícia Refojo Paulo Castro Andreia Silva

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