Resources for “South-South” Cooperation Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas Maceió, Alagoas State, Brazil Prof. Gilberto Fontes Prof a Eliana M. Mauricio da Rocha Lymphatic Filariasis Elimination in the Americas 5 th Regional Program Review Group (RPRG) Meeting (San José, Costa Rica, 27 October 2005)

2 Lymphatic Filariasis in Maceió

3 Centro de Ciências Biológicas Universidade Federal de Alagoas 3

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5 Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas History 1990 Survey of Army Soldiers Dr a Gerusa Dreyer & Staff: 2 mf +

6 Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas Epidemiological Study of LF in Maceió -Survey of microfilariae carriers (Maceió): 72 mf + (Students) -Distribution of LF endemic in Maceió: 3 city sectors -Distribution of LF in Alagoas State: Maceió -Epidemiological study of LF in Maceió: Human infection, entomological studies.

7 Maceió Endemic infectious diseases Small number of researchers Few Health PG programs Laboratory: Undergraduate students from Medicine, Pharmacy, Biology, Nursing, Nutrition Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas

8  Epidemiology  Entomology  Diagnostics 1990–2005: 525 undergraduate students, interns, fellows (CNPq / FAPEAL / UFAL / SMS)  Clinical Studies  Treatment  Health Education Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas

9 Part of the Trainees Team LF - Maceió LF - Maceió 9

10 Part of the Trainees Team LF - Maceió LF - Maceió 10

11 Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas Field Activities  Survey of the general population  Health information  Nighttime blood collection Finger prick Venous blood collection  Treatment and patient follow-up  Child Survey - ICT Card Test

12 Previous registry Thick blood smear Fieldwork 12

13 Microscopy examination Database EPI-info Membrane filtration 13

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18 Endemic area Canal (Reginaldo) Lymphatic Filariasis Laboratory Centro de Ciências Biológicas Universidade Federal de Alagoas

19 Surveys: Maceió, Alagoas 1999  2,821 examined21 microfilaremics(0.74%) 2000  12,669 examined69 microfilaremics(0.54%) 2001  13,544 examined66 microfilaremics(0.49%) 2002  24,159 examined23 microfilaremics(0.10%) 2003  7,450 examined 6 microfilaremics(0.08%) 2004  2005  6,715 examined 7,553 examined - 4 microfilaremics 0 microfilaremic (0.06%) (0.00%)

20 Collaborations -Fundação Nacional de Saúde (FUNASA) -Ministério da Saúde do Brasil / Secretaria da Vigilância em Saúde (MS/SVS) - Secretaria Municipal de Saúde de Maceió. LF Elimination Program - Centro de Pesquisas René Rachou, FioCruz. Standardization of Polymerase Chain Reaction (PCR)

21 Collaborations -Secretaria Estadual de Saúde de Belém. Thick Blood Smear Evaluation ( 2001–2003). Children’s ICT card. Xenomonitoring for PCR - Secretaria Estadual de Saúde da Bahia. Xenomonitoring for PCR (2005) - Centro de Pesquisas Aggeu Magalhães, FioCruz. Surveys and follow-up. Immunological studies for antigenemia

22 Collaborations - OPAS/OMS (PAHO/WHO-Brazil) -ICT card surveys -An integrated, community based approach in the control of soil transmitted worms -COMBI Project (Communication of Behavioural Impact) in LF area - IPK Cuba (Luis Fonte): PCR Entamoeba

23 Collaborations - PCR Leishmania and PCR Entamoeba -PhD thesis UFPE - Experimental antimalarial chemotherapy - Natural products Postgraduate Health Program at UFAL (September 2005)

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