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ÁCIDOS NUCLÉICOS Nucleotídeo Nucleotídeo Unidade Formadora de Ac. Nucléico.

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Apresentação em tema: "ÁCIDOS NUCLÉICOS Nucleotídeo Nucleotídeo Unidade Formadora de Ac. Nucléico."— Transcrição da apresentação:

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2 ÁCIDOS NUCLÉICOS

3 Nucleotídeo Nucleotídeo Unidade Formadora de Ac. Nucléico

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5 Bases Nitrogenadas: Purínicas: Adenina Guanina Pirimidínicas: Citosina Timina Uracila

6 Tipos: DNA (Ac. Desoxirribonucléico) RNA (Ac. Ribonucléico)

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9 C T G T C A C T C G G G T G A C AG T G A G C C C A DNA: ESTRUTURA GERAL

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11 DNA: ESTRUTURA DETALHADA

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13 Se a informação genética contida em cada célula nucleada fosse escrita em um livro,haveriam aproximadamente páginas escritas de ambos os lados AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT AATCGATCGATGCCTGT GACTCGATCGAAACTCG ACTCGCTCGCTAGATCG ACGTAGCACGACTAGCT CCGGGGCTCGAAAACTC TCTGACTACGATCGCTC GCGCTCGCTGGGGAATC CCGCTAGCAACATCGAT TCGCGCTAGCTACGCTA CCCCTTTGGAAATACAG GGTAATAGCGATCGATG GGATACAGATCGATCAT ATCGATCGGGAACGCGC CGATAATAAGGATAGCT

14 C T G T C A C T C G G G T G A C AG T G A G C C C A DNA: ESTRUTURA GERAL

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16 FLUXO DA EXPRESSÃO GÊNICA DNA RNA PROTEÍNA DNA

17 REPLICAÇÃO: Biossíntese de DNA à partir de um molde de DNA Ocorre na fase S Ocorre no sentido 5’ – 3´ Semi-conservativo

18 Começa em sítios específicos (Ricos em A e T Eucarioto: ~30.000sítios por cromossoma Procarioto: Único sítio (OriC)

19 Replicação: Contínua 5’ –3’ Descontínua (Fragmentos de OKASAKI)

20 COMPONENTES: Helicase SSB Primer Primase DNA Polimerase SIGMA Topoisomerase DNA Ligase

21 PCR (Polymerize Chain reaction) Replicação in vitro Aplicação: Diagnóstico molecular de Doenças Genéticas e Infecciosas

22 CONDIÇÔES DA PCR Temperatura: Desnaturação : 95 o C Anelamento: 60 o C Corrida: 72 o C Vários Ciclos: 30 Enzima: Taq Polimerase

23 T T C G...T T C G A T G G A T A A A C C G A... G G C T...A A G C T A C C T A T T T G G C T... Reação em Cadeia da Polimerase

24 T T C G...T T C G A T G G A T A A A C C G A... A A G C G G C T...A A G C T A C C T A T T T G G C T... C C G A C C G A

25 T T C G...T T C G A T G G A T A A A C C G A... G G C T...A A G C T A C C T A T T T G G C T... Reação em Cadeia da Polimerase T T C G...T T C G A T G G A T A A A C C G A... G G C T...A A G C T A C C T A T T T G G C T...

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