Emerson Siqueira Ministério da Educação Universidade Federal do Rio Grande do Norte Programa de Pós-Graduação em Ciências Farmacêuticas Disciplina: Sistemas.

Apresentação em tema: "Emerson Siqueira Ministério da Educação Universidade Federal do Rio Grande do Norte Programa de Pós-Graduação em Ciências Farmacêuticas Disciplina: Sistemas."— Transcrição da apresentação:

1 Emerson Siqueira Ministério da Educação Universidade Federal do Rio Grande do Norte Programa de Pós-Graduação em Ciências Farmacêuticas Disciplina: Sistemas de Liberação de Fármacos Docente: Prof. Dr. Arnóbio Antônio da Silva Júnior Impact factor: 8.387

2 2 NANODIAMONDS Ranging from 1 to 100 nm Chemical stability, large surface area and low costs of production Biotechnological and medical applications Carriers of biomolecules and drugs inside living cells and organisms High compatibility with cell Less toxic among nanocarbons  do not induce significant oxidative stress or apoptosis during cell treatments

3 3 EXPERIMENTAL DESIGN functionalized with chemotherapeutic molecules antineoplastic properties (in vitro and in vivo) Cell culture Cell culture: HeLa and murine melanoma cells Nanodiamonds were treated with three different protocols: o oxidation, ND; o wet chemical reduction, ND R1; o plasma reduction, ND R2. Aim of the project Aim of the project: nanodiamond potentialities as intracellular transporters of bioactive molecules  application for therapeutic purposes.

4 4 EXPERIMENTAL DESIGN penetration kinetics intracellular carriers of plant metabolites antiproliferative effects when on nanodiamonds different surface chemical structures of nanodiamonds Action mechanisms Action mechanisms: reactive oxygen species intracellular amount, cytotoxicity levels and induction of apoptosis.

5 5 CHARACTERIZATION

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11 Conclusions  i) nanodiamonds were able to penetrate in tumor cells, in a dose dependent manner, within 2 h of treatment;  ii) nanodiamonds did not enter in cell nuclear region but they remained embedded in nuclear membrane just exposing some little portions into nucleus;  iii) ciproten and quercetin antiproliferative effects were improved by nanodiamond conjugation;  iv) plant secondary metabolites highly reduced their in vitro pro-oxidant activity when functionalized with nanodiamond;  v) cytotoxic and pro-apoptotic properties of plant compounds were almost totally attenuated by linkage with nanodiamonds;  vi) nanodiamond-conjugated plant molecules would act at cellular level by different molecular mechanisms with respect to their pure forms;  vii) the effects of this therapeutic application on various tumor cell lines can be different;  viii) chemical and structural modifications of nanodiamond surfaces could influence biological effects of transported molecules; 11

12 siqueira_emerson@hotmail.com Ministério da Educação Universidade Federal do Rio Grande do Norte Programa de Pós-Graduação em Ciências Farmacêuticas Disciplina: Sistemas de Liberação de Fármacos Docente: Prof. Dr. Arnóbio Antônio da Silva Júnior Impact factor: 8.387