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PULSENET AMERICA LATINA 5 ta REUNION ANUAL 23 - 25 de octubre 2007 Buenos Aires, Argentina PFGE-PulseNet experience – 2006/2007 Activity plan – 2007/2008 São Paulo State, Brazil São Paulo State, Brazil Ana Terezinha Tavechio Instituto Adolfo Lutz São Paulo, Brazil
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EXPERIENCE 2006-2007 CHEF DR III acquisition BioNumerics acquisition Salmonella Gel analysis certification Outbreaks PFGE analysis
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Serotype Total Number of Outbreaks PFGE - Number of outbreaks / isolates S. Enteritidis33 6 / 14 S. Javiana1 1 / 2 S. Typhi1 1 / 5 S. Typhimurium1 1 / 7 S. Infantis1 1 / 6 S. I 1,9,12:-:- *1 1 / 6 Salmonella/Shigella Outbreaks analyzed by PFGE (PulseNet) – 2006-2007 Shigella sonnei11 / 9 * August 2007
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Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 8, 15, Braenderup H9812; Lanes 2-7, Infantis (2007); Lanes 9-11, Bareilly (2005); Lanes 12-14, Corvallis (2004) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5
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Salmonella Outbreaks in São Paulo State - Brazil Lanes 1,8,15, Braenderup H9812; Lanes 2-4, Typhimurium; Lanes 5-7, Typhimurium; Lanes 9-10, Javiana; Lanes 11-12, Typhi * (2007); Lanes 13-14, Tennessee * (foodstuff) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 * Esporadic cases 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5
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Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Lanes 1, 8, 15, Braenderup H9812; Lanes 2-4 and 5-7, S. Enteritidis (2007); Lanes 9-11 and 12-14*, S. Enteritidis (2006) * cake 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5
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Salmonella Outbreaks in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 Lanes 1, 10, Braenderup H9812; Lanes 2-6, Typhi (outbreak) Lanes 7-9, Typhi (sporadic cases) (2006) 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 KB
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Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7,8, S. enterica 9,12:-:- (August 2007); Lane 9, S. enterica 1,9,12:-:- (esporadic case – September 2007) 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 1 2 3 4 5 6 7 8 9 10
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Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7-9, Shigella sonnei (2007) * Lane 8, different PFGE and resistance profiles Shigella Outbreak in São Paulo State - Brazil 1 2 3 4 5 6 7 8 9 10 1135 668,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5
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2007 - 2008 Activity Plan PFGE analysis Outbreaks Extraintestinal Sporadic cases unusual serotypes Salmonella Gel analysis
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PulseNet database of Adolfo Lutz - SP Salmonella E. coli O157:H7 animal reservoirs Participation on the PulseNet Projects 2007 - 2008 Activity Plan
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Ana Terezinha Tavechio Instituto Adolfo Lutz Av. Dr. Arnaldo, 351 – 9º andar Cerqueira Cesar – São Paulo – SP CEP 01246.902 – Brasil atavechio@ial.sp.gov.br atavecch@hotmail.com THANK YOU!
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