Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables.

Apresentação em tema: "Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes João F. Mano 3B´s Research Group - Biomaterials, Biodegradables."— Transcrição da apresentação:

1 Engenharia de Tecidos Humanos: Um breve Resumo do Estado da Arte e Algumas Evoluções Recentes
João F. Mano 3B´s Research Group - Biomaterials, Biodegradables and Biomimetics, Dept. of Polymer Engineering, Campus de Gualtar, Braga, Portugal IBB- Institute for Biotechnology and Bioengineering, Braga, Portugal

2 3B´s RESEARCH GROUP Staff - Rui L. Reis (Director) - João F. Mano
- Nuno M. Neves - Natália Alves Other PhD Holders Alexandra Marques Ana Grenha Ana Frias António Salgado Carlos Viegas Erkan Baran Gabriela Silva Helena Azevedo Isabel Dias Iva Pashkuleva Johan Benesch Luciano Boesel Maria Manuela Gomes Mazen Tamer Ricardo Pires Rita Serra Rui Amandi de Sousa Sandra Sampaio Susana Martins Tiago Silva Zhongkui Hong PhD Students/ Other Full time Researchers Adriano Pedro Albino Martins Ana Mendes Ana Pinheiro Ana Oliveira Ana Sousa Ana Pinto Anabela Pinto Barbara Bertini Brecht Claes Bruno Ferreira Catarina Alves Cornelia Brunner Daniela Coutinho Elisabete Pinho Elizabeth Balmayor Emanuel Fernandes Helena Lima Isabel Leonor Ivo Aroso Jessica Grech Joana Magalhães João Oliveira Joaquim Ferreira Joaquim Oliveira José Rodriguez José Pereira José Júnior Kadriye Tuzlakoglu Leandro Gardel Márcia Rodrigues Marina Santos Marta Silva Patrícia Baptista Paula Sol Paula Pérez Paulo Bessa Ricardo Silva Rogério Sousa Rosa Bessada Sandra Osório Sangwong Chung Satyabrata Gosh Sílvia Pereira Simone Silva Susana Santos Teresa Silva Tírcia Santos Tommaso Rada Vitor Espírito Santo Vítor Silva Wojciech Szymczyk Technical Staff Ariana Santos Bárbara Barroso Berta Almeida Filipe Ribeiro Liliana Gomes Ricardo Gomes Tânia Delalande Alves Virgínia Araújo

3 TE: AN INTERDISCIPLINARY SCIENCE
“Tissue Engineering (TE) is an interdisciplinary field that applies the principles of engineering and the life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function” Langer and Vacanti., Science 260, 920 (1993) Biology Biochemistry Chemistry Materials Science & engineering Medical science TE

4 Biodegradable polymers Porous/interconected Which processing method??
CONSTRUCTION OF HUMAN TISSUE SUBSTITUTES What material?? Biodegradable polymers Which structure ?? Porous/interconected Which processing method?? SCAFFOLD cells + Growth factors

5 Starch + EVOH or CA or PCL or PLA
Materials used at the 3B’s Group Starch + EVOH or CA or PCL or PLA High potential for a all range of biomedical applications: - tissue engineering scaffolding - drug delivery carrier systems - bone replacement / bone defects; bone plates / screws; partially degradable bone cements Chitosan based materials Referring to polymeric materials, just a short list is presented here on polymers have been proposed for the construction of the scaffold in tissue engineering application, spitted into two major classes: synthetic polymers and natural based-ones. We believed that the combination of both types of polymers could be a possibility for combining the most favourable properties of synthetic and natural polymers, allowing to tailor the final materials properties and processability, together with the possibility to attach biologically functional groups that promote favourable cell-polymer interactions. other polysaccharides, proteins and blends with biodegradable polyesters…

6 3B´s SCAFFOLD PROCESSING ROUTES
Injection moulding with blowing agents Extrusion with blowing agents Microwave Baking Fiber Bonding (Wet or Melt-spinning) Compression moulding and salt leaching Solvent casting-particle leaching In-situ polymerization (In-situ) Enzymatic Degradation Freeze drying Rapid prototyping ……… Using all these materials our group have developed and optimize a very wide range of processing technologies to obtain scaffolds with different porous morphologies and different properties. Some of these methodologies were based in conventional processing technologies, such as injection molding and extrusion, and other were based on more non-conventional technologies. In the rest of the talk I will try to give some examples of how chitosan could be shaped into different forms.

7 ceramic+chitosan bilayered scaffolds
HA slurry pore size: 50 – 350 µm chitosan solution burning polyurethane sponge freeze-drying Just an example in this context combining ceramics and chitosan. First a polyurethane sponge is impregnated with a HA slurry. A ceramic scaffold may be then obtained after burning this ensemble. The scaffold is placed in a soft mould when a viscous chitosan solution is added into the top. After freeze drying a bilayer scaffold is obtained with the bottom layer being adequate to integrate with bone and the chitosan scaffold to the cartilage side. Studies are in progress to develop special bioreactor that may allow for the co-culturing of ostheoblasts and chondrocites in the two compartments.

8 STEM /PROGENITOR CELLS
EMBRYONIC BONE MARROW ADIPOSE TISSUE UMBILICAL CORD AMNIOTIC MEMBRANE

9 Cell response to bilayered scaffolds
20 m cartilage region condrogenic medium goat marrow cells 28 days 100 µm osteogenic medium Isabel Dias, Carlos Viegas bone region 14 days

10 Human Osteoblast-like cells (SaOs-2) and human fibroblasts (MRC-5)
Cell encapsulation and co-cultures + - Polyanion Ca2+ ions Cells Human Osteoblast-like cells (SaOs-2) and human fibroblasts (MRC-5) sodium alginate + Iota carrageenan + cells CaCl2+NaCl Fibroblasts Osteoblasts

11 INTERNALIZATION OF CMC/PAMAM-FITC NANOPARTICLES
SaOs-2 with CMC/PAMAM-FITC after 14 days novel carboxymethyl-chitosan/poly (amidoamine) (CMC/PAMAM) dendronized polymers (natural-based polymers + dendrimers). Slide 9 Falar que a solução oxidante foi abandonada e usou-se a solução ácida para preparar os materiais porosos.

12 NEW JOURNAL - TERM EU FUNDED PROJECTS Editor-in-Chief PROTEUS
STREP – WaCheUp IP-GENOSTEM NANOPHOTOTECH Marie Curie EST JoinTEd Marie Curie: POLYSTEM Marie Curie EST - ALEA JACTA EST STREP – HIPPOCRATES PROTEUS INTERREG IIIA NoE – EXPERTISSUES Marie Curie SCF - InVENTS NEW JOURNAL - TERM Editor-in-Chief Prof. Rui L. Reis

13 Biomaterials Biodegradables Biomimetics jmano@dep.uminho.pt
Concluding, we believe that natural-origin polymers may be a potential source of materials to be used in tissue engineering. However, much work is still needed, namely in controlling the chemical structure and the morphology at different length scales, to optimise the interaction between the scaffolds and the cells and tissues.