PULSENET AMERICA LATINA 5 ta REUNION ANUAL de octubre 2007 Buenos Aires, Argentina PFGE-PulseNet experience – 2006/2007 Activity plan – 2007/2008 São Paulo State, Brazil São Paulo State, Brazil Ana Terezinha Tavechio Instituto Adolfo Lutz São Paulo, Brazil

EXPERIENCE  CHEF DR III acquisition  BioNumerics acquisition  Salmonella Gel analysis certification  Outbreaks PFGE analysis

Serotype Total Number of Outbreaks PFGE - Number of outbreaks / isolates S. Enteritidis33 6 / 14 S. Javiana1 1 / 2 S. Typhi1 1 / 5 S. Typhimurium1 1 / 7 S. Infantis1 1 / 6 S. I 1,9,12:-:- *1 1 / 6 Salmonella/Shigella Outbreaks analyzed by PFGE (PulseNet) – Shigella sonnei11 / 9 * August 2007

Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 8, 15, Braenderup H9812; Lanes 2-7, Infantis (2007); Lanes 9-11, Bareilly (2005); Lanes 12-14, Corvallis (2004) ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5

Salmonella Outbreaks in São Paulo State - Brazil Lanes 1,8,15, Braenderup H9812; Lanes 2-4, Typhimurium; Lanes 5-7, Typhimurium; Lanes 9-10, Javiana; Lanes 11-12, Typhi * (2007); Lanes 13-14, Tennessee * (foodstuff) * Esporadic cases ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5

Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 8, 15, Braenderup H9812; Lanes 2-4 and 5-7, S. Enteritidis (2007); Lanes 9-11 and 12-14*, S. Enteritidis (2006) * cake ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5

Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 10, Braenderup H9812; Lanes 2-6, Typhi (outbreak) Lanes 7-9, Typhi (sporadic cases) (2006) ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5 KB

Salmonella Outbreaks in São Paulo State - Brazil Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7,8, S. enterica 9,12:-:- (August 2007); Lane 9, S. enterica 1,9,12:-:- (esporadic case – September 2007) ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,

Lanes 1, 6, 10, Braenderup H9812; Lanes 2-5 and 7-9, Shigella sonnei (2007) * Lane 8, different PFGE and resistance profiles Shigella Outbreak in São Paulo State - Brazil ,9 452,7 398,4 336,5 310,1 244,4 216,9 138,9 104,5 78,2 54,7 33,3 28,8 20,5

Activity Plan  PFGE analysis  Outbreaks  Extraintestinal Sporadic cases  unusual serotypes  Salmonella Gel analysis

 PulseNet database of Adolfo Lutz - SP  Salmonella  E. coli O157:H7 animal reservoirs  Participation on the PulseNet Projects Activity Plan

Ana Terezinha Tavechio Instituto Adolfo Lutz Av. Dr. Arnaldo, 351 – 9º andar Cerqueira Cesar – São Paulo – SP CEP – Brasil THANK YOU!