Parede Celular Vegetal Aternativas para o estudo da

Tamanho celular Formato das células Expansão Adesão Oligossacarídeos sinalizadores Formação de parede de aposição Têxtil Produção de papel Energia sustentável Por que estudar a Parede Celular Vegetal?

Exemplos de alternativas metodológicas

Mouille G. et al, The Plant Journal (2007); Krupková E. et al, The Plant Journal (2007)

Exemplos de alternativas metodológicas

Princípios da hibridação in situ

Exemplos de alternativas metodológicas

Overview of the key stages in the CoMPP technique PectinNon- cellulosicCellulose PectinNon- cellulosicCellulose Moller I. et al The Plant J (2007)

Quantificação, sensibilidade e reprodutibiliade do CoMPP  É preciso que cada amostra seja representada por vários spots  É necessário também minimizar o númeno de spots saturados

Análise da parede celular de Arabidopsis utilizando CoMPP Moller I. et al The Plant J (2007)

Links relacionados CarboSource Services (CCRC) CAZy - Carbohydrate-Active enZymes CAZypedia Cell Wall Genomics at Purdue Cell Wall Navigator at Riverside Complex Carbohydrate Research Centre Expansin Central Monoclonal antibodies for plant cell walls (CCRC) Paul Dupree's group at Cambridge Plantcellwall.org Plantcellwalls.net PlantProbes Stephen Fry's cell wall group at Edinburgh WallBioNet WallBioNet monoclonal antibody data base

Referências Bhandari, S. et al. Xylem-specific and tension stress-responsive coexpression of KORRIGAN endoglucanase and three secondary wall-associated cellulose synthase genes in aspen trees. Planta 224, (2006). Kalluri, U.C. & Joshi, C.P. Differential expression patterns of two cellulose synthase genes are associated with primary and secondary cell wall development in aspen trees. Planta 220, 47-55(2004). Krupková, E. et al. The TUMOROUS SHOOT DEVELOPMENT2 gene of Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development. The Plant Journal 50, (2007). Lionetti, V. et al. Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea. Plant physiology 143, (2007). Moller, I. et al. High-throughput mapping of cell-wall polymers within and between plants using novel microarrays. The Plant Journal 50, (2007). Mouille, G. et al. Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain. The Plant Journal 50, (2007). Pereira, L.a. et al. Deficiency of adenosine kinase activity affects the degree of pectin methyl-esterification in cell walls of Arabidopsis thaliana. Planta 224, (2006). Obrigado!