Legal Amazon Extra Amazon Manaus Wilson Duarte Alecrim

Apresentação em tema: "Legal Amazon Extra Amazon Manaus Wilson Duarte Alecrim"— Transcrição da apresentação:

1 Legal Amazon Extra Amazon Manaus Wilson Duarte Alecrim
Maria das Graças Alecrim Malaria Research in the Amazonas State FMT-AM Cuiabá Cor Jesus Fernandes Fontes Francisco Jose Dutra Souto Nucleus of Infectious and Tropical Diseases in Mato Grosso –UFMT Brasília Pedro Luiz Tauil João Barberino Santos Elisabeth Carmen Duarte Nucleus of Tropical Medicine-UFB Belo-Horizonte Antoniona U. Krettli Experimental and Human Malaria FIOCRUZ Luzia Helena Carvalho Luciano Andrade Moreira Malaria Molecular Biology and Immunology – FIOCRUZ Rio de Janeiro José Daniel Figueroa-Villar Luzineide Wanderley Tinoco Synthesis, Spectroscopy, Molecular Modeling and Biological Evaluation of Bioactive Compounds –IME Claudio T. Daniel Ribeiro Leonardo Carvalho Maria F. Ferreira da Cruz Centre for Malaria Research and Training Mariano Gustavo Zalis Malaria Molecular Biology –UFRJ Solange Wardell Isabela Ribeiro FIOCRUZ/Farmanguinhos DNDi - WHO São Paulo Hernando A. del Portillo Obando Maria Del Carmen F. Becerra Molecular Biology of Plasmodium-USP Alejandro Miguel Katzin Malaria Drug Resistance –USP Legal Amazon Extra Amazon

2 Synthesis and Natural Products
New Drugs Synthesis and Natural Products

3 Antoniana Krettli et al
Antoniana Krettli et al. Antimalarial activity of 4-(5-trifluoromethyl-1H-pyrazol-1-yl)-chloroquine analogues

4 Bidens pilosa, called “picão”,
Traditional medicine in Brazil, antimalarial tests and opportunities for malaria control Antoniana Ursine KRETTLI & M.Graça Lins BRANDÃO Bidens pilosa, called “picão”, Ampelozyziphus amazonicus, the “Indian beer”

5

6 Figueroa-Villar et al. Synthesis of Derivatives of the 2,4,6-triaminopirimidinas ()

7 Introduction of new drug regimens
Solange Wardell and coworkers and Isabela Ribeiro Development of an artesunate / mefloquine co-formulation The results of the 500-patient clinical study comparing AS/MQ with existing separate tablet formulations show a very high cure rate for patients in both groups – 94.1% for AS/MQ and 92.0% for the separate tablet formulation.

8 Assessment of philosseptin-1, a peptide isolated from the cutaneous secretion of Phyllomedusa hypochondrialis activity, and its activity on the P. falciparum growth. Carlos Eduardo Tosta

9 Cor Jesus Fernandes Fontes
Proguanil plus sulfamethoxazole in the treatment of uncomplicated Plasmodium falciparum malaria proguanil 100 mg BID plus sulfamethoxazole 1000 mg BID for 7 days

10 Drug resistance in P. vivax and P. falciparum
In vitro drug test methodologies for fresh field isolates. Molecular mechanisms of drug resistance

11 Evaluation of Plasmodium falciparum and P. vivax sensitivity
to antimalarials using the DELI-TEST Carvalho LJM; Chehuan-Mello Y, Ferreira-da-Cruz MF, Druilhe P; Alecrim WD, Brasseur P, Daniel-Ribeiro CT. Distribution of IC50 (in nMol/ml) for the different drugs assayed for P. falciparum and P. vivax

12 Molecular determinants of drug response and resistance in P
Molecular determinants of drug response and resistance in P. falciparum of Africa and South America. Mariano Zalis to understand the basis of resistance of P. falciparum to antimalarial drugs (including artemisinin) and explore potentials to improve the potency of existing antimalarial drugs such as CQ, AQ and QN.

13 Transporters PF-A0590w, PFC0125w, PF11-0225, PF13-0271, PF11-0466,
PFB0465c, PFL1125w PFL0620c PFE1185w, PF , PF , PFE0775c, PF , PF , PF , PFL1410c, PfATP6

14 In vitro sensitivity for Quinine
amino acid position ISOLATE ORIGIN 191 437 657 PVL558 Pt. Velho/BR MCP120 Macapá/BR RBR710 Rondônia/BR BEL307 Belém/BR BEL351 MCP155 MCP151 MCP114 MCP186 MCP189 MCP196 MCP197 MCP138 MCP116 MCP177 MCP191 SPO23 B.Ventura/CO SOP42 SOP38 SOP19 SNPs analysis of the PFA0590w gene and IC50 values to quinine in South America Increase IC50values to quinine Brasil IC50 range (38 nM – 257 nM) Brazil: nM Colombia: (38 – 147 nM) mutation wild type -3D7 Colômbia

15 Modulation of chloroquine sensitivity in P. falciparum by
over-expression of a P. vivax ortholog of the chloroquine resistance transporter gene, pfcrt Juliana M Sa, Marcio M Yamamoto, Carmen Fernandez-Becerra , Thomas E Wellems, Hernando A del Portillo