P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa Villunger, A. et al. Science, 2003 Flávia Gama Gomes Leite
p53 – ciclo celular e apoptose p53 p21 Parada do ciclo celular Mais de 16 genes alvos Apoptose ?
Possíveis Genes Alvos Puma (p53-upregulated modulator of apoptosis) Noxa (Noxius stress) Domínio BH3
Construção de Noxa -/-
Noxa -/- Northern blot Agarose - controle Southern blot PCR Southern blot
Noxa -/- Estímulo dependente de p53
Noxa -/- Estímulos independentes de p53 Estímulos dependentes de p53
Construção de Puma -/-
Puma -/- Southern blot PCR Southern blot - sonda exon 2 RT- PCR Northen blot
Puma -/- genotóxico
Independente de p53
Puma -/-