P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins.

Apresentação em tema: "P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins."— Transcrição da apresentação:

1 P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa Villunger, A. et al. Science, 2003 Flávia Gama Gomes Leite

2 p53 – ciclo celular e apoptose p53 p21 Parada do ciclo celular Mais de 16 genes alvos Apoptose ?

3 Possíveis Genes Alvos Puma (p53-upregulated modulator of apoptosis) Noxa (Noxius stress) Domínio BH3

4 Construção de Noxa -/-

5 Noxa -/- Northern blot Agarose - controle Southern blot PCR Southern blot

6 Noxa -/- Estímulo dependente de p53

7 Noxa -/- Estímulos independentes de p53 Estímulos dependentes de p53

8 Construção de Puma -/-

9 Puma -/- Southern blot PCR Southern blot - sonda exon 2 RT- PCR Northen blot

10 Puma -/- genotóxico

11 Independente de p53

12 Puma -/-

13